NanoTemper Dianthus

The NanoTemper Dianthus measures molecular binding affinity using NanoTemper’s Spectral Shift technology. The Dianthus uHTS is the ultra-high-throughput configuration, and Revolution drives both — the driver exposes a uHTS measurement and a classic measurement separately.

This is one of the more completely integrated instruments in Revolution: unlike most readers, the driver does not stop at triggering a measurement. It also retrieves the results.

At a glance

Plate formats	1536- or 384-well plates
Throughput (uHTS)	A full 1536-well plate in under 8 minutes
Data rate (uHTS)	Approximately 250,000 data points per day
Affinity range	Picomolar to millimolar
Detection	Spectral Shift — only one binding partner needs to be labelled
Revolution control	Run measurements, gate control, temperature, barcode read, experiment download and management

How the measurement works

NanoTemper states that Spectral Shift measurements are:

• In-solution and immobilisation-free
• Independent of mass changes upon binding
• Able to detect an interaction with only one binding partner labelled
• Collected without prior knowledge of the binding site

They describe the platform as compatible with any automation solution, supporting hours of uninterrupted, unattended operation — which is what makes the result-download operations below worth having.

Supported methods

Measuring

• Run uHTS measurement — the ultra-high-throughput measurement
• Run classic measurement — the standard Dianthus measurement
• Cancel measurement — abort the measurement in progress
• Wait for idle — block until the instrument is free, so a schedule can sequence around it
• Set temperature — set the measurement temperature

Plate access

• Open gate / Close gate — move the instrument’s gate so a robot can load and unload
• Get gate state — read whether the gate is open or closed before committing a move
• Read plate barcode — identify the plate on the instrument

Results and housekeeping

• List experiments — enumerate what is held on the instrument
• Download experiment / Download experiments / Download all experiments — retrieve result data to the configured download folder
• Rename experiment
• Delete experiments / Delete all experiments
• Check disk space — verify there is room before starting a run

The housekeeping operations are not incidental. A long unattended screening campaign generates data on the instrument, and an instrument that runs out of disk mid-campaign loses the run. Check disk space and the minimum free disk space setting exist so a schedule can fail early and loudly instead.

Integration

Revolution talks to the Dianthus over the network, addressing it by hostname and port.

Prerequisites

• The instrument must be reachable from the Revolution host at the configured hostname and port.
• The download folder must exist and be writable, and it needs enough room for the campaign you are about to run.
• The gate must be free to open — a robot’s approach path and the gate’s travel must not collide.

Device configuration

Property	Purpose
Hostname	Address of the instrument
Port	Port the instrument’s service listens on
Download Folder	Where retrieved experiment results are written
Auto Export Results	Export results automatically as measurements complete
Gate Timeout (s)	How long to wait for the gate to reach its commanded position
Measurement Timeout (min)	How long to wait for a measurement to complete before failing
Minimum Free Disk Space (MB)	Threshold below which the disk-space check fails
Connect In Simulation Mode	Run the driver without a physical instrument, for method development

Set the measurement timeout against the measurement you are actually running: a full 1536-well uHTS plate and a classic measurement are not the same duration, and a timeout tuned for one will cut the other short.

If you need more of this instrument driven from a schedule, get in touch — the driver is extended on demand.